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KMID : 0379120050330020059
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Korean Journal of Mycology
2005 Volume.33 No. 2 p.59 ~ p.63
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Favorable Condition of Culture and Sclerotial Formation by Inonotus obliquus
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Lee Won-Ho
Cha Ju-Young
Park Young-Jin
Kim Ho-Kyoung
Kim Tae-Woong
Sung Jae -Mo
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Abstract
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Altogether twenty isolates were collected from Wakayama, Japan. The optimum mycelial growth of I. obliquus was observed in BMYA and MCM. The optimum temperature and pH for the mycelial growth were $25^{\circ}C\;and\;6.0{\sim}7.0$, respectively. The optimum carbon and nitrogen sources were dextrose and yeast extract, respectively. Similarly, the optimum mineral salt was $K_{2}HPO_{4}$. The optimum number of mycelial discs for the mycelial growth was $6{\sim}7$ per 100 ml. Similarly, the optimum culture period was $21{\sim}22$ days in liquid broth. The optimum brown rice : water ratio was 1 : 1. No difference in mycelial growth was observed in all the four types of tree stumps used.
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KEYWORD
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Carbon sources, Inonotus obliquus, Nitrogen sources, Optimum culture period
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